Part:BBa_K1475003:Design

tetracyclin repressor from transposon Tn10

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

The part was constructed by PCR from the iGEM registry part TetR+LVA (Part:BBa_C0040) using the following primers, which are designed to remove the LVA rapid degradation tag:
Forward primer (containing prefix):
AAGAATTCGCGGCCGCTTCTAGATGTCCAGATTAGATAAAAGTAAAGTGATTAACAGCG
Reverse primer (containing suffix):
TTTCTGCAGCGGCCGCTACTAGTATTATTAGGACCCACTTTCACATTTAAGTTGTTTTTC

Source

Organizm: Escherichia coli

References

1. C. Krafft, et al.: Interaction of Tet Repressor with Operator DNA and with Tetracycline Studied by Infrared and Raman Spectroscopy. Biophysical Journal, Volume 74, Issue 1, January 1998, Pages 63–71. http://www.sciencedirect.com/science/article/pii/S0006349598777677
2. Tetsystems, 2008: Principles and Components Description. http://www.tetsystems.com/science-technology/principles-components